Thermo Fisher Scientific GABRA1 Monoclonal Antibody (N95/35), APC
GABRA1 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체로, APC로 표지되어 형광 분석에 적합합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 인간, 마우스, 랫트 시료에 반응합니다. 단백질 G로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N95/35 |
| Immunogen | Fusion protein amino acids 355–394 of human GABA-A-R-Alpha1 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931939 |
Additional Formats
- Unconjugated (MA5-27703)
- FITC (MA5-45486)
- PE (MA5-45488)
- PerCP (MA5-45487)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45485 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 55 kDa. No cross-reactivity against GABA-A-R-Alpha2 or -Alpha3.
Target Information
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing hyperpolarization of the membrane through the opening of a Cl⁻ channel associated with the GABA_A receptor (GABA_A-R) subtype.
GABA_A-Rs are important therapeutic targets for sedative, anxiolytic, and hypnotic agents and are implicated in diseases such as epilepsy, anxiety, depression, and substance abuse.
The GABA_A-R is a multimeric subunit complex consisting of multiple alpha, beta, and gamma subunits with various splice variants. Expression of alpha- and beta-subunits results in functional GABA_A-Rs, while coexpression of a gamma-subunit is required for benzodiazepine modulation.
Phosphorylation of beta-subunits has been shown to modulate receptor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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