Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), FITC
FITC 결합된 Occludin 단클론 항체로, 세포 간 밀착연접 단백질 분석에 적합합니다. WB, IHC, ICC/IF, Flow Cytometry 등에 사용 가능하며, 다양한 종에 반응합니다. 단백질 A로 정제된 고순도 항체로 안정적인 형광 검출을 제공합니다.
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Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 6 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OC-3F10 |
| Immunogen | GST fusion protein consisting of the C-terminal region (~150aa) of human occludin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533102 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 594
- HRP
- Request custom conjugation
Target Information
Occludin is a 65 kDa protein that can exist in various phosphorylated forms (up to ~82 kDa). It plays a key role in regulating tight junction localization and function. Polyunsaturated fatty acids up-regulate occludin expression, enhancing transendothelial resistance and reducing permeability. Expression levels vary by tissue—high in brain tissue and lower in non-neural tissues. Structural features are highly conserved across species. Under-expression of occludin contributes to tumor endothelial junction permeability and brain tumor edema formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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