Thermo Fisher Scientific GRLF1/p190RhoGAP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat and Dog

Target Information

RNA polymerase (Pol) III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors.
To maintain rapid growth, cells must produce a high level of Pol III-transcribed RNA, which requires TFIIIB and TFIIIC2 transcription factor complexes.
The TFIIIC2 complex (TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, TFIIIC63) is overexpressed in malignant cells such as ovarian carcinomas and recruits RNA Pol III and TFIIIB to promoter elements.
The TFIIIB complex includes TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, which are also upregulated in transformed cells.
In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to regulate TFIIIB function.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.