Thermo Fisher Scientific Vimentin Polyclonal Antibody
Vimentin 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며 인간 및 마우스 반응성을 가집니다. 항원 친화 크로마토그래피로 정제된 액상 시약으로 안정적 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human VIM. Recombinant protein control fragment (Product #RP-100898). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789129 |
Product Specific Information
Immunogen sequence:
FANYIDKVRF LEQQNKILLA ELEQLKGQGK SRLGDLYEEE MRELRRQVDQ LTNDKARVEV ERDNLAEDIM RLREKLQEEM LQREEAENTL QSFRQDVDNA SLARLDLERK VES
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal changes that facilitate cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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