Thermo Fisher Scientific PGP9.5 Recombinant Rabbit Monoclonal Antibody (012)
Rat UCHL1 단백질을 인식하는 recombinant rabbit monoclonal antibody로, 높은 특이성과 재현성을 제공합니다. Western blot, ELISA, Immunoprecipitation에 적합하며, preservative-free 포뮬레이션으로 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 012 |
| Immunogen | Recombinant Rat UCHL1 protein (Glu2–Ala223) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785692 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
This antibody has specificity for Rat UCHL1.
Target Information
PGP9.5 (Protein Gene Product 9.5, UCH-L1, PARK5) is a neuron-specific protein, structurally and immunologically distinct from neuron-specific enolase. It has a molecular weight of approximately 27 kDa and belongs to the ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12).
Key characteristics:
- Exhibits ubiquitin hydrolase and ligase activities
- Present in neurons, nerve fibers, neuroendocrine cells, renal tubules, spermatogonia, Leydig cells, and corpus luteum
- Overexpression linked to non-small cell lung cancer
- Decreased expression associated with Huntington’s and Alzheimer’s diseases
PGP9.5 serves as a useful neuronal marker and is valuable in studies of neurodegenerative disorders such as Parkinson’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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