Thermo Fisher Scientific p47phox Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 258 of Human NCF1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p47phox,uniProtId:P14598-1,ncbiNodeId:9606,antigenRange:1-258,antigenLength:390,antigenImageFileName:PA5-27821_p47phox_P14598-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27821_p47phox_P14598-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545297

Product Specific Information

Recommended positive controls: Raji.

Predicted reactivity: Human (99%), Mouse (83%), Rat (80%), Pig (82%), Rabbit (86%), Bovine (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.