Thermo Fisher Scientific Phospho-eNOS (Ser114) Polyclonal Antibody
eNOS의 Ser114 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 농도의 액상 시약으로, -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NOS3 (Accession P29474), corresponding to amino acid residues around phosphorylated Ser114. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2903138 |
Product Specific Information
This antibody detects endogenous levels of eNOS only when phosphorylated at Ser114.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that functions as a signaling molecule between cells. It plays roles in vasorelaxation, neurotransmission, and cytotoxicity. NO production is mediated by nitric oxide synthase (NOS) enzymes, which catalyze the oxidation of L-arginine to produce L-citrulline and NO. Three NOS isoforms have been cloned:
- nNOS (neuronal, type I)
- iNOS (inducible, type II)
- eNOS (endothelial, type III)
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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