Thermo Fisher Scientific Phospho-Tau (Ser717, Ser400) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000–1:3,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Ser717/400.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, functioning as a linker between both structures. Axonal polarity is influenced by TAU/MAPT localization in the neuronal cell body domain defined by the centrosome.

Short isoforms of Tau contribute to cytoskeletal plasticity, whereas longer isoforms aid stabilization. In its hyper-phosphorylated form, Tau becomes the major component of paired helical filaments (PHF), which are the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brains. Hyper-phosphorylation disrupts Tau’s microtubule-binding ability, leading to microtubule destabilization and neuronal degeneration in AD.

Tau phosphorylation is mediated by several serine/threonine kinases, including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is also found in neurofibrillary lesions associated with other central nervous system disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.