Thermo Fisher Scientific AKAP3 Polyclonal Antibody
Human AKAP3 단백질을 인식하는 염소 유래 폴리클로날 항체로 Western blot에 적합합니다. 합성 펩타이드 면역원으로 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 단기 4°C, 장기 -20°C 보관이 권장됩니다.
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Thermo Fisher Scientific AKAP3 Polyclonal Antibody
Applications
- Western Blot (WB): Tested dilution 1–3 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide TPLQLLDWLMVNL, corresponding to C-terminal amino acids 841–853 of Human AKAP3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2273888 |
Product Specific Information
- Recommended positive control: The peptide used to generate this antibody is available for purchase (GTX19046-PEP).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase within the cell.
AKAP3 (also known as AKAP110, FSP95, PRKA3, SOB1) binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions.
Expression of AKAP3 is restricted to testis tissue, where bicarbonate stimulates tyrosine phosphorylation of AKAP3, increasing its recruitment of PKA.
AKAP3 also exhibits high expression in epithelial ovarian cancer (EOC) and is associated with worse overall survival, making it a potential target for antigen-specific immunotherapy in EOC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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