Thermo Fisher Scientific E2F5 Monoclonal Antibody (OTI3B7), TrueMAB
E2F5 단백질을 인식하는 단클론 항체로 인간, 마우스, 랫트에 반응합니다. Western blot에 적합하며, 동결건조 형태로 제공됩니다. PBS 버퍼에 8% 트레할로스가 포함되어 있으며 방부제는 없습니다. 세포주기 조절 연구 및 종양 억제 단백질 연구에 활용됩니다.
- 판매단위
- pk
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Applications
- Western Blot (WB): 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B7 |
| Immunogen | Full length human recombinant protein of human E2F5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
E2F5 is a member of the E2F family of transcription factors that regulate the cell cycle and interact with tumor suppressor proteins. The E2F family contains conserved domains, including:
- DNA binding domain
- Dimerization domain (interaction with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain
E2F5 is differentially phosphorylated and expressed in various human tissues. It shares high sequence identity with E2F4 and interacts with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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