Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of human platelets.
Recommended use: 5 µL (1 µg) per test in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).

Spectral Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD42b is a platelet activation marker involved in coagulation as an aggregating factor. It interacts with extracellular matrix and adhesion molecules. CD42b expression and activation are regulated by VEGF and PDGF.
It forms part of the GPIb-IX-V receptor complex, critical for platelet-rich thrombus formation by tethering platelets to thrombogenic surfaces. CD42b binds to von Willebrand factor (VWF), thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1, and P-selectin.
The extracellular domain contributes to metastasis. CD42b antigen is expressed on platelets and megakaryocytes. Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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