Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), PE-Cyanine5, eBioscience
인간 CD11c를 인식하는 마우스 단클론 항체로, PE-Cyanine5 형광 표지된 유세포분석용 시약입니다. 단핵구, 대식세포, NK 및 수지상세포 분석에 적합합니다. 광민감성이 있으므로 어둡게 보관하며, 4°C에서 냉장 보관합니다.
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- 15-0116-42
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | PE-Cyanine5 |
| Excitation / Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573054 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subsets of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesive interactions.
Applications Reported:
This 3.9 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.06 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test (empirically determined).
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm excitation; 667 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD11, along with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, and eosinophils.
- CD11b: Expressed by monocytes, macrophages, NK cells, some B and T-cells, and granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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