Thermo Fisher Scientific Mycoplasma Arginine Deiminase Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Bacteria

Expression System

E. coli

Amino acid sequence

SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSAILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK DKLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKTDLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RETLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGVSE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWLTMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQS IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYSRNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW

Molecular weight

46.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 97% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G1 and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.