Thermo Fisher Scientific CENPH Monoclonal Antibody (OTI2G4), TrueMAB
CENPH 단백질을 표적하는 Mouse IgG1 단클론 항체로, WB, IHC(P), Flow Cytometry에 사용 가능. 인간 시료에 반응하며, 동결건조 형태로 제공. 고순도 친화 크로마토그래피 정제 제품으로 연구용에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G4 |
| Immunogen | Full length human recombinant protein of human CENPH produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Centromere and kinetochore proteins are essential for centromere structure, kinetochore formation, and sister chromatid separation.
CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C during interphase and metaphase.
It is localized outside of centromeric heterochromatin (where CENP-B resides) and inside the kinetochore corona (where CENP-E localizes during prometaphase).
CENPH can bind to itself and to CENP-A, CENP-B, or CENP-C. Multimers of CENPH are constitutively localized to the inner kinetochore plate and play a key role in organizing and functioning of the active centromere-kinetochore complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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