Thermo Fisher Scientific WT1 Recombinant Rabbit Monoclonal Antibody (6L9X6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6L9X6

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WT1,uniProtId:P19544-1,ncbiNodeId:9606,antigenRange:60-250,antigenLength:449,antigenImageFileName:MA5-42786_WT1_P19544-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42786_WT1_P19544-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911927

Product Specific Information

Positive test controls include: Rat testis. The target is usually found in the following locations: Cytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm.

Immunogen sequence: PPPPPPPPPH SFIKQEPSWG GAEPHEEQCL SAFTVHFSGQ FTGTAGACRY GPFGPPPPSQ ASSGQARMFP NAPYLPSCLE SQPAIRNQGY STVTFDGTPS YGHTPSHHAA QFPNHSFKHE DPMGQQGSLG EQQYSVPPPV YGCHTPTDSC TGSQALLLRT PYSSDNLYQM TSQLECMTWN QMNLGATLKG V

Target Information

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.