Thermo Fisher Scientific WT1 Recombinant Rabbit Monoclonal Antibody (6L9X6)
WT1 단백질을 인식하는 recombinant rabbit monoclonal antibody로, Western blot, IHC, ELISA에 적합합니다. 인간, 마우스, 랫트 시료에서 반응하며, 고순도 affinity chromatography로 정제되었습니다. -20°C에서 안정적으로 보관 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6L9X6 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 60–250 of human WT1 (P19544) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911927 |
Product Specific Information
- Positive test controls include: Rat testis.
- Localization: Cytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm.
- Immunogen sequence:
PPPPPPPPPH SFIKQEPSWG GAEPHEEQCL SAFTVHFSGQ FTGTAGACRY GPFGPPPPSQ ASSGQARMFP NAPYLPSCLE SQPAIRNQGY STVTFDGTPS YGHTPSHHAA QFPNHSFKHE DPMGQQGSLG EQQYSVPPPV YGCHTPTDSC TGSQALLLRT PYSSDNLYQM TSQLECMTWN QMNLGATLKG V
Target Information
The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic RanGAP is critical for establishing a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer nuclear surface in metazoan and plant cells. Arabidopsis thaliana RanGAP association with the nuclear envelope requires plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 represent a second family of CC-TMD proteins, structurally similar but distinct from WIPs, required for RanGAP nuclear envelope association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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