Thermo Fisher Scientific DDX3 Monoclonal Antibody (3B9E3)
DDX3 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3B9E3). Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer에 sodium azide 포함.
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Thermo Fisher Scientific DDX3 Monoclonal Antibody (3B9E3)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3B9E3 |
| Immunogen | Purified recombinant fragment of human DDX3X (aa: 518–661) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898422 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
The DDX3X protein is a member of the DEAD-box protein family characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and exhibits ATP-dependent RNA helicase activity. It displays high RNA-independent ATPase activity, stimulated by both RNA and DNA. DDX3X functions in both the nucleus and cytoplasm, participating in transcriptional regulation, mRNP assembly, pre-mRNA splicing, mRNA export, translation, cellular signaling, and viral replication. Dysregulation of this gene is associated with tumorigenesis. A paralog, DDX3Y, is located in the nonrecombining region of the Y chromosome, and pseudogenes similar to both DDX3X and DDX3Y exist on chromosome 4 and the X chromosome. Alternative splicing leads to multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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