Thermo Fisher Scientific Nrf2 Polyclonal Antibody
Nrf2 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC, ICC/IF 등 다양한 면역실험에 적용 가능. Human, Mouse 반응성 확인됨. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 저장용액에 1% BSA 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to C-terminus of human Nrf2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.21 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2108511 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is placenta or lung tissue.
Target Information
Nfr1 (also designated TCF11), Nrf2, and Nfr3 are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE (Maf recognition elements) sequences.
Nrf2 is widely expressed and translocates to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from the repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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