
Thermo Fisher Scientific UBE1 Recombinant Rabbit Monoclonal Antibody (SD08-62)
Thermo Fisher Scientific의 UBE1 Recombinant Rabbit Monoclonal Antibody (SD08-62)는 인간, 생쥐, 랫트 반응성을 가지며 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC, Flow Cytometry에 적합하며, 재조합 기술을 통해 일관된 품질을 보장합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD08-62 |
| Immunogen | Synthetic peptide within Human UBA1 aa 1,009–1,058 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809680 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Key advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It complements an X-linked mouse temperature-sensitive defect in DNA synthesis, possibly functioning in DNA repair.
Located on chromosome Xp11.23, UBE1 has two transcript variants encoding the same protein but with different 5’ UTRs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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