Thermo Fisher Scientific DARC Chimeric Recombinant Rabbit Monoclonal Antibody (2C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2C3

Immunogen

CHO cells transfected with cDNA encoding the Duffy glycoprotein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074548

Product Specific Information

Specificity: The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.

Target Information

ACKR1 or DARC is an atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR1 is also known as an interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. It has a promiscuous chemokine-binding profile by interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. ACKR1 also acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and for the malaria parasites P.vivax and P.knowlesi.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.