Thermo Fisher Scientific IARS Polyclonal Antibody
Human 및 Rat에서 반응하는 Rabbit Polyclonal IARS 항체로, WB, IHC, IP에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태. 293T, HeLa 등 다양한 세포에서 양성 대조 가능하며 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IARS (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735944 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2
- Predicted Reactivity: Mouse (92%), Xenopus laevis (80%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IARS is a member of aminoacyl-tRNA synthetases that catalyze the aminoacylation of tRNA by their cognate amino acid. These enzymes play a central role in linking amino acids with nucleotide triplets contained in tRNAs and are considered among the earliest proteins in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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