Thermo Fisher Scientific HSP27 Polyclonal Antibody
HSP27 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC, Flow, IP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제. 연구용 전용 제품.
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Thermo Fisher Scientific HSP27 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP27 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735924 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2, HT-29, Mouse liver
- Predicted Reactivity: Dog (86%), Pig (86%), Rhesus Monkey (100%), Bovine (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In response to environmental stress, cells increase expression of heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein functioning as a molecular chaperone to maintain denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its chaperone activity, HSP27 regulates biological processes including phosphorylation and axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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