Thermo Fisher Scientific Phospho-HSF1 (Ser121) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation upstream of heat shock genes. Although HSEs are conserved among species, HSFs from yeast, Drosophila, and human differ in molecular weight and immunological cross-reactivity.

Two HSFs (HSF1 and HSF2) have been identified in human cells, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 becomes activated to a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is essential for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.