Thermo Fisher Scientific LIGHT Monoclonal Antibody (1F4A5)
인간 CD258(LIGHT) 단백질을 표적으로 하는 마우스 단클론 항체로 다양한 면역실험(WB, ICC/IF, Flow, ELISA)에 사용 가능. 단백질 G 정제, 액상 형태, 1 mg/mL 농도. 연구용으로만 사용되며 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F4A5 |
| Immunogen | Purified recombinant fragment of human CD258 (AA: 59–240) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090774 |
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family.
A novel DD-containing molecule, Daxx (death associated protein 6), was cloned from mouse, human, and monkey. Daxx binds specifically to the Fas death domain, enhances Fas-induced apoptosis, and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult tissues, indicating its important role in Fas signaling pathways.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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