Thermo Fisher Scientific SQSTM1 Recombinant Rabbit Monoclonal Antibody (PS00-61)
SQSTM1/p62 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, HEK293 세포에서 발현된 recombinant IgG 형식입니다. 단백질 A로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PS00-61 |
| Immunogen | Synthetic peptide within Human SQSTM1/p62 aa 400 to the C-terminus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094157 |
Product Specific Information
- Positive control: PC-12 cell lysate, RAW264.7 cell lysate, PANC-1 cell lysate, mouse liver tissue lysate, rat brain tissue lysate, SH-SY5Y cell lysate, HeLa cell lysate, Hela-si-NT cell lysate, Hela-si-p62#1 cell lysate, Hela-si-p62#2 cell lysate, human colon carcinoma tissue, A549.
- Predicted band size: 48 kDa
- Subcellular Location: Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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