Thermo Fisher Scientific H3K9ac Polyclonal Antibody
H3K9ac 폴리클로날 항체로 Western blot, IHC, ICC, ELISA, IP, ChIP 등 다양한 응용에 사용 가능. 사람, 마우스, 랫트 반응성. Rabbit IgG 기반으로 0.4 mg/mL 농도의 액상 형태. PBS 버퍼와 50% 글리세롤 포함, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:20–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human Histone H3 (NP_003520.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901722 |
Product Specific Information
- Positive samples: HeLa, NIH/3T3, C6
- Immunogen sequence: ARTKQTARKS TGGKAPRK
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histone H3 plays a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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