Thermo Fisher Scientific Desmoglein 3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Desmoglein 3. Validated for WB and ELISA. Recognizes cell junction and membrane-localized protein. Supplied as liquid, affinity purified, stored in PBS with glycerol. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 749–999 of human Desmoglein 3 (NP_001935.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914766 |
Product Specific Information
- Positive test controls: HeLa, Mouse liver
- Localization: Cell junction, Cell membrane, Single-pass type I membrane protein, Desmosome
Immunogen sequence:
FGAATGVGIC SSGQSGTMRT RHSTGGTNKD YADGAISMNF LDSYFSQKAF ACAEEDDGQE ANDCLLIYDN EGADATGSPV GSVGCCSFIA DDLDDSFLDS LGPKFKKLAE ISLGVDGEGK EVQPPSKDSG YGIESCGHPI EVQQTGFVKC QTLSGSQGAS ALSTSGSVQP AVSIPDPLQH GNYLVTETYS ASGSLVQPST AGFDPLLTQN VIVTERVICP ISSVPGNLAG PTQLRGSHTM LCTEDPCSRL I
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominantly expressed in stratified squamous epithelium such as skin, oral mucosa, and esophagus. Desmoglein 3 is the autoantigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies leads to the loss of cell-cell adhesion, resulting in blistering of the skin and oral mucosa and histologically in suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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