Thermo Fisher Scientific MDM2 Recombinant Rabbit Monoclonal Antibody (3N0U4)
Recombinant rabbit monoclonal antibody against human MDM2 (clone 3N0U4). Validated for WB and ELISA. High specificity and reproducibility. Suitable for research on p53 regulation and tumor biology. For research use only.
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- MA555290
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- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MDM2 Recombinant Rabbit Monoclonal Antibody (3N0U4)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3N0U4 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 101–200 of human MDM2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Immunogen Sequence:
TMIYRNLVVV NQQESSDSGT SVSENRCHLE GGSDQKDLVQ ELQEEKPSSS HLVSRPSTSS RRRAISETEE NSDELSGERQ RKRHKSDSIS LSFDESLALC
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting p53 ubiquitination and degradation. Phosphorylation also prevents MDM2 binding to p19ARF, further enhancing p53 degradation. Overexpression of MDM2 can inactivate p53, reducing its tumor suppressor function. MDM2 also participates in apoptosis and cell cycle regulation and is overexpressed in various human cancers, including soft tissue carcinomas and breast cancer. It interacts with proteins such as retinoblastoma 1 and ribosomal protein L5. Over 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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