Invitrogen™

BLOCK-iT™ U6 RNAi Entry Vector Kit

The BLOCK-iT™ U6 RNAi Entry Vector provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing자세히 알아보기

The BLOCK-iT™ U6 RNAi Entry Vector provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi), a preferred technique for analyzing gene downregulation. An easy cloning process places an ∼50-bp oligonucleotide DNA immediately following a U6 pol III type promoter (Figure 1). Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA (siRNA) that will generate the RNAi effect.

*Delivery of the U6 RNAi Cassette
*Once cloning is complete, the U6 Entry Vector is ready to be used immediately in transient transfections using a reagent such as Lipofectamine™ 2000. This makes this system ideal for initial shRNA screenings in many mammalian cell types. As an alternative in hard-to-transfect or non-dividing cell types, or to deliver to animal model systems, the RNAi cassette can easily be recombined into a BLOCK-iT™ Viral RNAi vector. For stable shRNA lentiviral delivery and expression, recombine the BLOCK-iT™ U6 Entry Vector with the pLenti6/BLOCK-iT™ RNAi Vector. For transient delivery to challenging cell types using adenoviral transduction, recombine with the pAd/BLOCK-iT™ RNAi Vector.

사양

제품 유형RNAi Entry Vector Kit

클로닝 방법Gateway™

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

RNAi 유형shRNA

벡터BLOCK-iT RNAi Vectors

프로모터U6

형식Kit

제품라인BLOCK-iT™

수량20 constructions

Unit SizeEach