Invitrogen™

pLenti6/V5 Directional TOPO™ Cloning Kit

The pLenti6⁄V5 Directional TOPO™ Cloning Kit contains the TOPO™-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO™ for quick PCR-based cloning and high-level자세히 알아보기

The pLenti6⁄V5 Directional TOPO™ Cloning Kit contains the TOPO™-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• High efficiency and rapid cloning
• Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system

Key Features
• Directional TOPO™ Cloning site for rapid and efficient directional cloning of blunt-end PCR products
• Rouse Sarcoma Virus (RSV) enhancer⁄promoter for Tat-independent production of viral mRNA in the producer cell line
• Modified HIV-1 5and 3 Long Terminal Repeats (LTR) for viral packaging and reverse transcription
• HIV-1 psi (ψ) packaging sequence for viral packaging
• HIV Rev response element (RRE) for Rev-dependent nuclear export of unspliced viral mRNA
• (CMV) immediate early promoter for high-level constitutive expression of the gene of interest in mammalian cells
• C-terminal V5 epitope for detection of the recombinant protein
• Blasticidin (bsd) resistance gene for selection in E. coli and mammalian cells
• Ampicillin resistance gene for selection in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli

Kit includes
pLenti6⁄V5-D- TOPO™ Reagents
One Shot™ Stbl3™ Chemically Competent E. coli

For research use only. Not intended for any therapeutic or diagnostic use.

사양

클로닝 방법Directional TOPO™

구성 또는 유도성 시스템Constitutive

배달 유형Lentiviral

용도(애플리케이션)Viral Expression

반응 수20 reactions

제품라인TOPO™, ViraPower™

제품 유형TOPO Cloning Kit

프로모터CMV

단백질 태그V5 Epitope Tag

수량20 Reactions

선택 제제(진핵)Blasticidin

벡터pLenti, Directional TOPO Vectors

Unit SizeEach