Thermo Fisher Scientific BLOCK-iT Lentiviral RNAi Expression System

Invitrogen™ BLOCK-iT™ Lentiviral RNAi Expression System

The pLenti6/BLOCK-iT™-DEST expression vector provided in the BLOCK-iT™ Lentiviral RNAi Expression System enables efficient introduction and stable expression of short hairpin RNA (shRNA) in vivo using a lentiviral vector.

A novel cloning process places an approximately 50-bp DNA oligonucleotide immediately downstream of a U6 pol III promoter in the BLOCK-iT™ U6 entry vector. The oligonucleotide expresses RNA forming a stem-loop structure containing sense and antisense regions of the target gene. This shRNA is recombined into the pLenti6/BLOCK-iT™-DEST vector.

After viral production and transduction, the U6 promoter-driven shRNA becomes stably integrated as an RNAi cassette. The generated shRNA avoids host defense mechanisms and effectively induces RNAi-mediated gene knockdown.

Vector Features

• attR sites for efficient recombination with attL-flanked U6 Gateway™ entry vector containing the RNAi cassette
• Includes all necessary components for efficient lentiviral packaging and delivery
• Blasticidin selection marker for rapid establishment of stable shRNA-expressing cell lines

Using this system, long-term gene silencing analysis can be performed in both dividing and non-dividing mammalian cells and animal models.

The BLOCK-iT™ RNAi U6 Entry Vector Kit allows streamlined cloning of shRNA target sequences for transient testing. Selected RNAi cassettes are efficiently recombined from the entry vector into the pLenti6/BLOCK-iT™-DEST vector via a standard Gateway™ LR recombination reaction.

사양 (Specifications)

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