Thermo Fisher Scientific MHC Class I Recombinant Rat Monoclonal Antibody (R1-21.2)
마우스 MHC Class I에 특이적인 재조합 Rat 단클론 항체로, IHC, IP, RIA 등 다양한 면역분석에 사용 가능. HEK293 세포 발현, Protein A 정제, PBS 버퍼에 1 mg/mL 농도로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Immunohistochemistry (IHC)
- Tested Dilution: Assay-dependent
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Radioimmune Assays (RIA)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | R1-21.2 |
| Immunogen | Lewis 1F rats immunised with mouse CH-1 lymphoma cells; spleen cells fused with X-63Ag8-653 myeloma cells to generate hybridomas |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody is specific for murine MHC class I and binds to polymorphic H-2 determinants expressed on most haplotypes. It binds all H-2 class I molecules, independent of allele, and inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
Target Information
Like their mammalian counterparts, avian MHC Class I molecules (B-F antigens) consist of a highly polymorphic alpha-chain noncovalently bound to the invariant beta2-microglobulin subunit. MHC Class I molecules are expressed on most nucleated cells, presenting endogenous antigenic peptides to CD8+ cytotoxic T lymphocytes.
The monoclonal antibody F21-2 also reacts with turkey MHC Class I.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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