Thermo Fisher Scientific IL-17F Monoclonal Antibody (SHLR17), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific IL-17F Monoclonal Antibody (SHLR17), Brilliant Violet 421, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F, a homodimeric 34 kDa member of the IL-17 family. IL-17F and IL-17A are produced by Th17 cells and have overlapping but distinct functions.
IL-17F binds to IL-17RA/IL-17RC heterodimer receptors, inducing secretion of pro-inflammatory cytokines and chemokines and recruiting neutrophils.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and validated using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).

A test is defined as the antibody amount that stains a cell sample in a final volume of 100 µL. Recommended cell number: 10⁵–10⁸ cells/test.

Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers equipped with a violet (405 nm) laser.
For panels using multiple polymer dyes, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75).

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

IL-17F (Interleukin 17F, CTLA-8) is a cytokine produced by inflammatory cells such as activated T cells, mast cells, and basophils. It plays a role in allergic airway inflammation and induces cytokines, chemokines, and adhesion molecules in various cell types.

IL-17F acts through IL-17R to recruit monocytes and neutrophils, stimulating TNFα, IL-1β, IL-6, and IL-8 production. It is implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.

IL-17F is produced as a homodimer and may form heterodimers with IL-17A. It inhibits angiogenesis and induces endothelial cells to produce IL-2, TGF-β, and MCP-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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