Thermo Fisher Scientific HRAS Monoclonal Antibody (OTI1H3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:200–1:4000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended.
(Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882)

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to various downstream signaling pathways that lead to distinct cytoplasmic or nuclear events.
Each mammalian cell contains three Ras proto-oncogenes coding for closely related Ras proteins: H-, K-, and N-Ras.
Oncogenic mutations in Ras genes are present in approximately 30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibits apoptosis.

• K-Ras mutations: Common in pancreatic, colorectal, and non-small-cell lung carcinomas
• H-Ras mutations: Common in bladder, kidney, and thyroid carcinomas
• N-Ras mutations: Found in melanoma, hematological malignancies, and hepatocellular carcinoma

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.