Thermo Fisher Scientific HRAS Monoclonal Antibody (OTI2A1), TrueMAB
HRAS 단백질 검출용 마우스 단일클론 항체로 Western blot, IHC, ICC/IF에 적합. 인간, 마우스, 개 시료 반응. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. Ras 신호전달 연구 및 암 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A1 |
| Immunogen | Full-length human recombinant HRAS protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HRAS is a monomeric G protein functioning as a molecular switch that links receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways.
Mammalian cells express three Ras proto-oncogenes (H-, K-, N-Ras), encoding closely related proteins.
Oncogenic Ras mutations occur in approximately 30% of human cancers, leading to uncontrolled proliferation and reduced apoptosis.
- K-Ras mutations: common in pancreatic, colorectal, and non-small-cell lung carcinomas
- H-Ras mutations: common in bladder, kidney, and thyroid carcinomas
- N-Ras mutations: found in melanoma, hematological malignancies, and hepatocellular carcinoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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