Thermo Fisher Scientific GSDMD Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 154-252 of human GSDMD (NP_079012.3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GSDMD,uniProtId:P57764-1,ncbiNodeId:9606,antigenRange:154-252,antigenLength:484,antigenImageFileName:PA5-116815_GSDMD_P57764-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116815_GSDMD_P57764-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.25 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901446

Product Specific Information

Positive Samples: PC-3, THP-1, Jurkat

Immunogen sequence: GDNVYVVTEV LQTQKEVEVT RTHKREGSGR FSLPGATCLQ GEGQGHLSQK KTVTIPSGST LAFRVAQLVI DSDLDVLLFP DKKQRTFQPP ATGHKRSTS

Target Information

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.