Thermo Fisher Scientific CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience

Applications and Tested Dilutions

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Product Specifications

Available Conjugates

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 690
• NovaFluor Yellow 570
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio84-3C1 reacts with CD43, also known as leukosialin, galactoglycoprotein, and sialophorin. CD43 is a sialomucin that can exhibit both adhesive and anti-adhesive functions. It is expressed on most leukocytes except resting B lymphocytes, and its surface expression decreases upon activation due to proteolytic processing. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, expressed on macrophages.

Applications Reported:
This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometry, western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Applications Tested:
Tested by flow cytometric analysis of human peripheral blood cells and by IHC of formalin-fixed paraffin-embedded human spleen using low pH antigen retrieval at ≤20 µg/mL. Optimal dilution should be determined by the user.

Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on most hematopoietic cells. It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation. Through interactions with actin-binding proteins such as ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte trafficking. CD43 signaling modulates LFA-1 adhesiveness and influences tumor suppressor p53 activity. Dysregulation of CD43 is associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.