Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650

Applications

• Flow Cytometry (Flow): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450–660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Functional Grade
• Custom conjugation available

Product Specific Information

• Description: Monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on normal human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome: Brilliant Violet™ 650 (BV650) emits at 649 nm; use with violet (405 nm) laser-equipped cytometers.
• Compatibility: Not compatible with methanol-based fixation.
• Recommended Buffers:
  • Super Bright Complete Staining Buffer (Product # SB-4401-42)
  • Brilliant Stain Buffer™ (Product # 00-4409-75)
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
• Laser/Emission: Excitation 407 nm; Emission 649 nm; Violet Laser.
• Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company; Powered by Sirigen™.

Target Information

Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a role in:

• Opsonization and complement activation
• Antibody-dependent cell-mediated cytotoxicity
• Regulation of immune responses (T cell activation, B cell inhibition)

Widely used in:

• ELISA, Western blotting, immunohistochemistry
• Immunotherapy and vaccine development

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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