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Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650, eBioscience
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Brilliant Violet 650으로 결합된 Mouse IgG2b kappa 단클론 동형 대조항체. Flow Cytometry용으로 검증됨. 비특이적 결합 평가 및 대조 실험에 적합. 407/649 nm 파장에서 형광 검출 가능. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 416-4732-81
- 판매단위
- pk
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Thermo Fisher Scientific 416-4732-81 Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650, eBioscience 50 ug pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650, eBioscience
Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 650
Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | Brilliant Violet™ 650 |
| Excitation/Emission Max | 407 / 646 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_3074124 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450–660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780
- Functional Grade
- Custom conjugation available
Product Specific Information
- Description: Monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on normal human peripheral blood cells; use at same concentration as experimental antibody.
- Fluorochrome: Brilliant Violet™ 650 (BV650) emits at 649 nm; use with violet (405 nm) laser-equipped cytometers.
- Compatibility: Not compatible with methanol-based fixation.
- Recommended Buffers:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
- Light Sensitivity: Protect from light to prevent photo-induced oxidation.
- Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
- Laser/Emission: Excitation 407 nm; Emission 649 nm; Violet Laser.
- Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company; Powered by Sirigen™.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a role in:
- Opsonization and complement activation
- Antibody-dependent cell-mediated cytotoxicity
- Regulation of immune responses (T cell activation, B cell inhibition)
Widely used in:
- ELISA, Western blotting, immunohistochemistry
- Immunotherapy and vaccine development
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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