Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

• Description: Monoclonal mouse IgG1 kappa immunoglobulin useful as an isotype control.
• Applications Reported: Reported for use in flow cytometric analysis.
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome Information: Brilliant Violet™ 650 (BV650) emits at 649 nm and is intended for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.
• Staining Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Sensitive to photo-induced oxidation; protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) up to 3 days at 4°C in dark.
• Compatibility: Not compatible with methanol-based fixation.
• Excitation / Emission / Laser: 407 nm / 649 nm / Violet Laser.

Trademark Notice: BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G antibody produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in adaptive immunity. It participates in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. Widely used in research and diagnostics such as ELISA, Western blotting, and immunohistochemistry, as well as in immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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