Thermo Fisher Scientific AIF Polyclonal Antibody
Thermo Fisher Scientific의 AIF 폴리클로날 항체는 인간, 마우스, 랫트 시료에서 AIF 단백질을 검출하기 위한 고순도 항체입니다. Western blot, IHC, ICC/IF 실험에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 61–110 of Human AIF-M1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803229 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Apoptosis Inducing Factor (AIF) is involved in chromatin condensation and DNA fragmentation during apoptosis. It localizes in mitochondria and translocates to the nucleus upon apoptotic induction, promoting release of apoptogenic proteins such as cytochrome c and caspase-9. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene are associated with combined oxidative phosphorylation deficiency 6, leading to mitochondrial encephalomyopathy. Alternative splicing produces multiple transcript variants and a pseudogene on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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