Thermo Fisher Scientific GRK2 Polyclonal Antibody
GRK2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능. 인간 및 생쥐 반응성. 고순도 항원 친화 크로마토그래피로 정제된 액상 형태.
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- PA527480
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 477–689 of Human GRK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.82 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544956 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, Jurkat, NCI-H929
- Predicted reactivity: Mouse (98%), Rat (98%), Pig (98%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ADRBK1 (GRK2) is a serine/threonine kinase. Many G protein-coupled receptors (GPCRs) are phosphorylated by G protein-dependent receptor kinases (GRKs). GRK2 (83 kDa), one of six members of this family, is ubiquitously expressed in mammals. After ligand binding and interaction with heterotrimeric G proteins, GPCRs (e.g., α2-adrenergic receptor) are phosphorylated by GRKs. Internalization of GPCRs, regulated by beta-arrestin-1, activates the Ras–Raf–ERK1&2 signaling pathway. GRK2 activity is controlled by phosphorylation (PKC, Src, ERK1&2) and protein interactions. ERK phosphorylates and inactivates GRK2 on serine 670 via a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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