Thermo Fisher Scientific PKP3 Monoclonal Antibody (23E3/4)
PKP3 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ELISA 등 다양한 분석에 적합합니다. 인간 및 마우스 시료에서 반응하며, 고순도 Protein A 정제 방식으로 제조되었습니다. 세포막-핵 신호전달 연구용으로 이상적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 6 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 3 publications |
| Immunocytochemistry (ICC/IF) | – | View 3 publications |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
| ChIP assay (ChIP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23E3/4 |
| Immunogen | Synthetic peptide from Armadillo domain of human Plakophilin-3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533221 |
Target Information
Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein is largely restricted to epithelial cell types. It can be detected along cell borders in a punctate staining pattern typical for desmosomal proteins. In addition to desmosomal localization, immunostaining shows bright nuclear speckles. Like plakophilin-1 and -2, plakophilin-3 displays dual intracellular localization in the desmosomal plaque and nucleus, suggesting involvement in signal transduction between plasma membrane and nucleus. The human protein has a predicted molecular mass of approximately 87 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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