Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test

Product Specifications

Available Conjugate Formats

• Alexa Fluor 561, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 737, 805
• Brilliant Violet 421, 480, 605, 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, and binds glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1, and MAdCam-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.125 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize titration for specific assays.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm, for use with a 405 nm violet laser. Ensure cytometer compatibility.

Staining Buffer Recommendation:
When using multiple polymer dyes (Super Bright, Brilliant Violet™, Brilliant UV™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light; this tandem dye is sensitive to photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).
Brilliant Violet™ 650 is not compatible with methanol-based fixation.

Excitation / Emission: 407 nm / 649 nm
Laser: Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:

• C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer, transmembrane, and cytoplasmic regions

CD62L is constitutively expressed on most leukocytes, including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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