Thermo Fisher Scientific HSF1 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation upstream of all heat shock genes.

Two HSFs have been identified in human cells—HSF1 and HSF2—which share 38% sequence identity and bind to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Upon stress, HSF1 becomes activated, forming a nuclear-localized trimeric state that binds DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.