Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Red 685, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Available Formats

• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• Custom conjugation available upon request

Product Specific Information

Description:
The MEM-15 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through surface CD14.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested for flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 4 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen™ LIVE/DEAD™ Fixable Dead Cell stains for viability analysis.
• Each NovaFluor conjugate or kit is shipped with CellBlox™ Blocking Buffer.
  • Add 5 µL per sample to antibody cocktails or single-color controls (10³–10⁸ cells).
  • Mix CellBlox Blocking Buffer with antibody cocktails before adding to cells.

Optical Properties:

• Excitation: 637 nm
• Emission: 685 nm
• Recommended Laser: 633–640 nm (Red)

Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with reduced spillover and spread.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils.
It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms.
Soluble CD14 (sCD14) can distinguish structural differences in lipopolysaccharides and contributes to immune modulation.
CD14 also binds apoptotic cells and is involved in allergic, inflammatory, and infectious processes.
Alternative splicing produces multiple transcript variants encoding the same isoform.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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