Thermo Fisher Scientific ASH2L Monoclonal Antibody (OTI7D11)
Human ASH2L 단백질(aa 347-628)에 특이적인 Mouse Monoclonal Antibody (Clone OTI7D11). Western blot 및 IHC(P)에서 1:2,000 희석으로 검증됨. Unconjugated 형태, 1 mg/mL 농도, PBS/BSA/glycerol buffer에 보관. -20°C에서 보관하며 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7D11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 347–628 of ASH2L produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723922 |
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), containing a nuclear localization signal and PHD finger motif, suggesting its role as a transcription regulator.
Predicted molecular weight: 68.7 kDa.
Alternative splicing may result in isoforms at 60 kDa and 56.5 kDa.
Human ASH2L shares 60% homology with Drosophila ash2, which regulates gene expression during early development, and 90% homology with mouse ASH2L.
ASH2L is predominantly expressed in adult heart, testis, and fetal lung and liver, with low expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
It is also expressed in leukemia cell lines (K562, Hel, Dami) with erythroid and megakaryocytic potential.
Differentiation inducers such as phorbol ester and hemin alter ASH2L expression patterns, indicating its involvement in hematopoiesis and leukemia.
Additionally, ASH2L has been reported to interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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