Thermo Fisher Scientific ASH2L Monoclonal Antibody (OTI2H3)

Applications

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:2,000

Product Specifications

Target Information

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), containing a nuclear localization signal and PHD finger motif, suggesting a role as a transcription regulator.
Predicted molecular weight: 68.7 kDa.
Alternative splicing may produce isoforms of 60 kDa and 56.5 kDa.

Human ASH2L proteins are:

• 60% homologous to Drosophila ash2, which regulates gene expression in early development.
• 90% homologous to mouse ASH2L.

Expression profile:

• Predominantly in adult heart and testis, and fetal lung and liver.
• Barely detectable in adult lung, liver, kidney, prostate, and peripheral leukocytes.
• Expressed in leukemia cell lines (K562, Hel, Dami) with erythroid and megakaryocytic potential.

Differentiation inducers (e.g. phorbol ester, hemin) alter expression patterns, indicating ASH2L’s role in hematopoiesis and leukemia association.
ASH2L may interact with beta-tubulin and beta-catenin.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.