Thermo Fisher Scientific Phospho-c-Fos (Thr325) Polyclonal Antibody
인산화된 c-Fos (Thr325)을 인식하는 토끼 폴리클로날 항체입니다. Western blot, ICC/IF, ChIP 등에 사용 가능합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
| ChIP assay (ChIP) | 1–3 µg × 10⁶ cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Fos that contains threonine 325. The sequence is conserved in human, mouse, and rat. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533622 |
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli, including growth factors and insulin, at multiple sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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