Thermo Fisher Scientific HA Tag Polyclonal Antibody, eBioscience
HA 태그 인식용 래빗 폴리클로날 항체로 다양한 단백질 검출 및 면역분석에 활용 가능. Western blot, IP, IF 등 여러 응용에 적합하며 높은 순도와 안정성 제공. 연구용으로 단백질 위치 확인 및 상호작용 분석에 유용.
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Thermo Fisher Scientific HA Tag Polyclonal Antibody, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View publications |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with BSA, 0.02% gelatin |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468300 |
Product Specific Information
The polyclonal antibody reacts with HA tag.
The HA tag is a nine amino acid peptide sequence (YPYDVPDYA) derived from the human influenza virus hemagglutinin protein. It has been successfully used in various applications such as Western blotting, immunoprecipitation, and immunofluorescence studies.
Applications Reported:
Purified Anti-HA tag poly has been reported for use in immunoprecipitation and immunoblotting (WB).
Applications Tested:
Purified Anti-HA tag poly has been tested by immunoblotting (WB) at a starting dilution of 1:200. It is recommended to titrate for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag used to localize gene products, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are not available.
HA is derived from the influenza hemagglutinin surface glycoprotein and is commonly expressed with recombinant proteins to facilitate identification and purification for functional analysis.
HA tag antibodies enable protein detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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