Thermo Fisher Scientific Phospho-alpha B Crystallin (Ser59) Polyclonal Antibody
Phospho-alpha B Crystallin (Ser59) 폴리클로날 항체로 인간, 마우스, 랫트에 반응합니다. 다양한 응용 분야(WB, IHC, ICC, Flow, ELISA)에 사용 가능하며, KLH 결합 합성 인산화 펩타이드 면역원으로 제작되었습니다. 단백질 A 정제, 액상 형태, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human alpha B Crystallin around phosphorylation site Ser59 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural role is to maintain the proper refractive index.
The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.
- Alpha-crystallin: largest class, composed of alpha-A and alpha-B gene products.
- Beta-crystallin: at least five different proteins.
- Gamma-crystallin: monomeric, with several variants identified in bovine and rat lens.
Alpha-Crystallin makes up about 40% of total lens protein. It functions as a molecular chaperone preventing aggregation that may lead to cataract formation. Phosphorylation of alpha-crystallin occurs in response to cellular stress and may play a role in structural regulation and protein maintenance.
Alpha-B crystallin has been associated with Alexander’s disease, where it accumulates in brain cells of affected individuals.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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