Thermo Fisher Scientific Phospho-MARK Pan (Thr215) Polyclonal Antibody
MARK1/2/3/4 단백질의 Thr215 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin) 실험에 최적화. 고순도 액상 형태로 제공되며, PBS·glycerol buffer에 보존. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MARK1 (Accession Q9P0L2), corresponding to amino acid residues around phosphorylated Thr215 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817213 |
Product Specific Information
Antibody detects endogenous levels of MARK1/2/3/4 only when phosphorylated at Thr215.
Target Information
MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases.
MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability.
MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a (Homo sapiens).
EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability, and cancer.
Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.
MARK3 was originally identified as a marker induced by DNA-damaging agents, and loss of MARK3 was found in pancreatic carcinogenesis.
MARK4 contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1-associated kinase domain.
RT-PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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