Thermo Fisher Scientific Phospho-MARK Pan (Thr215) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Mouse, Rat, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MARK1(Accession Q9P0L2), corresponding to amino acid residues around phosphorylated Thr215. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARK Pan,uniProtId:P27448-5,ncbiNodeId:9606,antigenRange:211|215,antigenLength:753,antigenImageFileName:PA5-105814_MARK_Pan_P27448-5_Rabbit.svg,antigenImageFileNamePDP:PA5-105814_MARK_Pan_P27448-5_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:MARK Pan,uniProtId:Q7KZI7-1,ncbiNodeId:9606,antigenRange:208|215,antigenLength:788,antigenImageFileName:PA5-105814_MARK_Pan_Q7KZI7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105814_MARK_Pan_Q7KZI7-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:MARK Pan,uniProtId:Q9P0L2-1,ncbiNodeId:9606,antigenRange:215,antigenLength:795,antigenImageFileName:PA5-105814_MARK_Pan_Q9P0L2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105814_MARK_Pan_Q9P0L2-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2817213

Product Specific Information

Antibody detects endogenous levels of MARK1/2/3/4 only when phosphorylated at Thr215.

Target Information

MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. MARK3 was originally identified as a marker that was induced by treatment with DNA damaging agents, and loss of MARK3 was found with carcinogenesis in the pancreas. MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.